Single-channel multiplexing without melting curve analysis in real-time PCR

Multiplex real-time PCR with quantification of targets in a single fluorescence channel has been the demand in biotechnology industry. Here, we develop a novel analytical real-time PCR technique to detect multiple targets in a single fluorescence channel without melting curve analysis. In this techn...

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Detalles Bibliográficos
Autores principales: Lee, Young-Jo, Kim, Daeyoung, Lee, Kihoon, Chun, Jong-Yoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262828/
https://www.ncbi.nlm.nih.gov/pubmed/25501038
http://dx.doi.org/10.1038/srep07439

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262828/
https://www.ncbi.nlm.nih.gov/pubmed/25501038
http://dx.doi.org/10.1038/srep07439

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