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Single-channel multiplexing without melting curve analysis in real-time PCR
Multiplex real-time PCR with quantification of targets in a single fluorescence channel has been the demand in biotechnology industry. Here, we develop a novel analytical real-time PCR technique to detect multiple targets in a single fluorescence channel without melting curve analysis. In this techn...
Autores principales: | Lee, Young-Jo, Kim, Daeyoung, Lee, Kihoon, Chun, Jong-Yoon |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262828/ https://www.ncbi.nlm.nih.gov/pubmed/25501038 http://dx.doi.org/10.1038/srep07439 |
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