Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9

Genome engineering using programmable nucleases enables homologous recombination (HR)-mediated gene knock-in. However, the labour used to construct targeting vectors containing homology arms and difficulties in inducing HR in some cell type and organisms represent technical hurdles for the applicati...

Descripción completa

Detalles Bibliográficos
Autores principales: Nakade, Shota, Tsubota, Takuya, Sakane, Yuto, Kume, Satoshi, Sakamoto, Naoaki, Obara, Masanobu, Daimon, Takaaki, Sezutsu, Hideki, Yamamoto, Takashi, Sakuma, Tetsushi, Suzuki, Ken-ichi T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263139/
https://www.ncbi.nlm.nih.gov/pubmed/25410609
http://dx.doi.org/10.1038/ncomms6560

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263139/
https://www.ncbi.nlm.nih.gov/pubmed/25410609
http://dx.doi.org/10.1038/ncomms6560

Ejemplares similares