Cargando…

Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9

Genome engineering using programmable nucleases enables homologous recombination (HR)-mediated gene knock-in. However, the labour used to construct targeting vectors containing homology arms and difficulties in inducing HR in some cell type and organisms represent technical hurdles for the applicati...

Descripción completa

Detalles Bibliográficos
Autores principales:	Nakade, Shota, Tsubota, Takuya, Sakane, Yuto, Kume, Satoshi, Sakamoto, Naoaki, Obara, Masanobu, Daimon, Takaaki, Sezutsu, Hideki, Yamamoto, Takashi, Sakuma, Tetsushi, Suzuki, Ken-ichi T.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Pub. Group 2014
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263139/ https://www.ncbi.nlm.nih.gov/pubmed/25410609 http://dx.doi.org/10.1038/ncomms6560

Ejemplares similares

Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity
por: Sakuma, Tetsushi, et al.
Publicado: (2013)

CRISPR/Cas‐based precision genome editing via microhomology‐mediated end joining
por: Van Vu, Tien, et al.
Publicado: (2020)

Microhomology Selection for Microhomology Mediated End Joining in Saccharomyces cerevisiae
por: Lee, Kihoon, et al.
Publicado: (2019)

Efficient CRISPR/Cas9‐based plant genomic fragment deletions by microhomology‐mediated end joining
por: Tan, Jiantao, et al.
Publicado: (2020)

Microhomology-mediated end joining: new players join the team
por: Wang, Hailong, et al.
Publicado: (2017)

Efficient TALEN Construction for Bombyx mori Gene Targeting
por: Takasu, Yoko, et al.
Publicado: (2013)

Correction: Efficient TALEN Construction for Bombyx mori Gene Targeting
por: Takasu, Yoko, et al.
Publicado: (2013)

Endogenous CRISPR-assisted microhomology-mediated end joining enables rapid genome editing in Zymomonas mobilis
por: Sui, Xin, et al.
Publicado: (2021)

High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos
por: Suzuki, Ken-ichi T., et al.
Publicado: (2013)

Homology and enzymatic requirements of microhomology-dependent alternative end joining
por: Sharma, S, et al.
Publicado: (2015)

Microhomology-mediated end joining induces hypermutagenesis at breakpoint junctions
por: Sinha, Supriya, et al.
Publicado: (2017)

The Expression of TALEN before Fertilization Provides a Rapid Knock-Out Phenotype in Xenopus laevis Founder Embryos
por: Miyamoto, Kei, et al.
Publicado: (2015)

Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems
por: Yasue, Akihiro, et al.
Publicado: (2014)

In vivo and in vitro knockout system labelled using fluorescent protein via microhomology-mediated end joining
por: Katayama, Shota, et al.
Publicado: (2019)

Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9
por: Sato, Takuya, et al.
Publicado: (2015)

Recurrent deletions in clonal hematopoiesis are driven by microhomology-mediated end joining
por: Feldman, Tzah, et al.
Publicado: (2021)

Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish
por: Hisano, Yu, et al.
Publicado: (2015)

Cas9, Cpf1 and C2c1/2/3―What's next?
por: Nakade, Shota, et al.
Publicado: (2017)

Mechanism of Microhomology-Mediated End-Joining Promoted by Human DNA Polymerase Theta
por: Kent, Tatiana, et al.
Publicado: (2015)

Robust activation of microhomology-mediated end joining for precision gene editing applications
por: Ata, Hirotaka, et al.
Publicado: (2018)

PLK1 targets CtIP to promote microhomology-mediated end joining
por: Wang, Hailong, et al.
Publicado: (2018)

Screening Methods to Identify TALEN-Mediated Knockout Mice
por: Nakagawa, Yoshiko, et al.
Publicado: (2014)

Highly efficient biallelic genome editing of human ES/iPS cells using a CRISPR/Cas9 or TALEN system
por: Takayama, Kazuo, et al.
Publicado: (2017)

Establishment of expanded and streamlined pipeline of PITCh knock-in – a web-based design tool for MMEJ-mediated gene knock-in, PITCh designer, and the variations of PITCh, PITCh-TG and PITCh-KIKO
por: Nakamae, Kazuki, et al.
Publicado: (2017)

Synthesis-dependent microhomology-mediated end joining accounts for multiple types of repair junctions
por: Yu, Amy Marie, et al.
Publicado: (2010)

A role for human homologous recombination factors in suppressing microhomology-mediated end joining
por: Ahrabi, Sara, et al.
Publicado: (2016)

Molecular basis of microhomology-mediated end-joining by purified full-length Polθ
por: Black, Samuel J., et al.
Publicado: (2019)

Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9
por: Li, Hongmei Lisa, et al.
Publicado: (2014)

Microhomology-assisted scarless genome editing in human iPSCs
por: Kim, Shin-Il, et al.
Publicado: (2018)

TALEN and CRISPR/Cas Genome Editing Systems: Tools of Discovery
por: Nemudryi, A. A., et al.
Publicado: (2014)

Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish
por: Xiao, An, et al.
Publicado: (2013)

CtIP promotes microhomology-mediated alternative end-joining during class switch recombination
por: Lee-Theilen, Mieun, et al.
Publicado: (2010)

Assays for DNA double-strand break repair by microhomology-based end-joining repair mechanisms
por: Kostyrko, Kaja, et al.
Publicado: (2016)

53BP1 promotes microhomology-mediated end-joining in G1-phase cells
por: Xiong, Xiahui, et al.
Publicado: (2015)

Plant organellar DNA polymerases repair double-stranded breaks by microhomology-mediated end-joining
por: García-Medel, Paola L, et al.
Publicado: (2019)

Polθ promotes the repair of 5′-DNA-protein crosslinks by microhomology-mediated end-joining
por: Chandramouly, Gurushankar, et al.
Publicado: (2021)

Biased genome editing using the local accumulation of DSB repair molecules system
por: Nakade, Shota, et al.
Publicado: (2018)

Plant genome editing with TALEN and CRISPR
por: Malzahn, Aimee, et al.
Publicado: (2017)

Establishment of CRFK cells for vaccine production by inactivating endogenous retrovirus with TALEN technology
por: Shimode, Sayumi, et al.
Publicado: (2022)

Microhomology-mediated end joining drives complex rearrangements and overexpression of MYC and PVT1 in multiple myeloma
por: Mikulasova, Aneta, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_evqc38kc9nb9g5bh91448u4lil