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Unexpected Transcellular Protein Crossover Occurs During Canonical DNA Transfection

Transfection of DNA has been invaluable for biological sciences, yet the effects upon membrane homeostasis are far from negligible. Here, we demonstrate that Neuro2A cells transfected using Lipofectamine LTX with the fluorescently coupled Botulinum serotype A holoenzyme (EGFP-LcA) cDNA express this...

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Detalles Bibliográficos
Autores principales: Arsenault, Jason, Cuijpers, Sabine AG, Niranjan, Dhevahi, Davletov, Bazbek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263260/
https://www.ncbi.nlm.nih.gov/pubmed/25043607
http://dx.doi.org/10.1002/jcb.24884