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Microfluidic-Based Amplification-Free Bacterial DNA Detection by Dielectrophoretic Concentration and Fluorescent Resonance Energy Transfer Assisted in Situ Hybridization (FRET-ISH) (†,‡)

Although real-time PCR (RT-PCR) has become a diagnostic standard for rapid identification of bacterial species, typical methods remain time-intensive due to sample preparation and amplification cycle times. The assay described in this work incorporates on-chip dielectrophoretic capture and concentra...

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Detalles Bibliográficos
Autores principales: Packard, Michelle M., Shusteff, Maxim, Alocilja, Evangelyn C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263560/
https://www.ncbi.nlm.nih.gov/pubmed/25586031
http://dx.doi.org/10.3390/bios2040405