Cargando…

Detection of DNA Sequences Refractory to PCR Amplification Using a Biophysical SERRS Assay (Surface Enhanced Resonant Raman Spectroscopy)

The analysis of ancient or processed DNA samples is often a great challenge, because traditional Polymerase Chain Reaction – based amplification is impeded by DNA damage. Blocking lesions such as abasic sites are known to block the bypass of DNA polymerases, thus stopping primer elongation. In the p...

Descripción completa

Detalles Bibliográficos
Autores principales: Feuillie, Cécile, Merheb, Maxime M., Gillet, Benjamin, Montagnac, Gilles, Daniel, Isabelle, Hänni, Catherine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4264738/
https://www.ncbi.nlm.nih.gov/pubmed/25502338
http://dx.doi.org/10.1371/journal.pone.0114148