Compact and Blinking-Suppressed Quantum Dots for Single-Particle Tracking in Live Cells

[Image: see text] Quantum dots (QDs) offer distinct advantages over organic dyes and fluorescent proteins for biological imaging applications because of their brightness, photostability, and tunability. However, a major limitation is that single QDs emit fluorescent light in an intermittent on-and-o...

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Autores principales: Lane, Lucas A., Smith, Andrew M., Lian, Tianquan, Nie, Shuming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4266335/
https://www.ncbi.nlm.nih.gov/pubmed/25157589
http://dx.doi.org/10.1021/jp5064325
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author Lane, Lucas A.
Smith, Andrew M.
Lian, Tianquan
Nie, Shuming
author_facet Lane, Lucas A.
Smith, Andrew M.
Lian, Tianquan
Nie, Shuming
author_sort Lane, Lucas A.
collection PubMed
description [Image: see text] Quantum dots (QDs) offer distinct advantages over organic dyes and fluorescent proteins for biological imaging applications because of their brightness, photostability, and tunability. However, a major limitation is that single QDs emit fluorescent light in an intermittent on-and-off fashion called “blinking”. Here we report the development of blinking-suppressed, relatively compact QDs that are able to maintain their favorable optical properties in aqueous solution. Specifically, we show that a linearly graded alloy shell can be grown on a small CdSe core via a precisely controlled layer-by-layer process, and that this graded shell leads to a dramatic suppression of QD blinking in both organic solvents and water. A substantial portion (>25%) of the resulting QDs does not blink (more than 99% of the time in the bright or “on” state). Theoretical modeling studies indicate that this type of linearly graded shell not only can minimize charge carrier access to surface traps but also can reduce lattice defects, both of which are believed to be responsible for carrier trapping and QD blinking. Further, we have evaluated the biological utility of blinking-suppressed QDs coated with polyethylene glycol (PEG)-based ligands and multidentate ligands. The results demonstrate that their optical properties are largely independent of surface coatings and solvating media, and that the blinking-suppressed QDs can provide continuous trajectories in live-cell receptor tracking studies.
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spelling pubmed-42663352015-08-26 Compact and Blinking-Suppressed Quantum Dots for Single-Particle Tracking in Live Cells Lane, Lucas A. Smith, Andrew M. Lian, Tianquan Nie, Shuming J Phys Chem B [Image: see text] Quantum dots (QDs) offer distinct advantages over organic dyes and fluorescent proteins for biological imaging applications because of their brightness, photostability, and tunability. However, a major limitation is that single QDs emit fluorescent light in an intermittent on-and-off fashion called “blinking”. Here we report the development of blinking-suppressed, relatively compact QDs that are able to maintain their favorable optical properties in aqueous solution. Specifically, we show that a linearly graded alloy shell can be grown on a small CdSe core via a precisely controlled layer-by-layer process, and that this graded shell leads to a dramatic suppression of QD blinking in both organic solvents and water. A substantial portion (>25%) of the resulting QDs does not blink (more than 99% of the time in the bright or “on” state). Theoretical modeling studies indicate that this type of linearly graded shell not only can minimize charge carrier access to surface traps but also can reduce lattice defects, both of which are believed to be responsible for carrier trapping and QD blinking. Further, we have evaluated the biological utility of blinking-suppressed QDs coated with polyethylene glycol (PEG)-based ligands and multidentate ligands. The results demonstrate that their optical properties are largely independent of surface coatings and solvating media, and that the blinking-suppressed QDs can provide continuous trajectories in live-cell receptor tracking studies. American Chemical Society 2014-08-26 2014-12-11 /pmc/articles/PMC4266335/ /pubmed/25157589 http://dx.doi.org/10.1021/jp5064325 Text en Copyright © 2014 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Lane, Lucas A.
Smith, Andrew M.
Lian, Tianquan
Nie, Shuming
Compact and Blinking-Suppressed Quantum Dots for Single-Particle Tracking in Live Cells
title Compact and Blinking-Suppressed Quantum Dots for Single-Particle Tracking in Live Cells
title_full Compact and Blinking-Suppressed Quantum Dots for Single-Particle Tracking in Live Cells
title_fullStr Compact and Blinking-Suppressed Quantum Dots for Single-Particle Tracking in Live Cells
title_full_unstemmed Compact and Blinking-Suppressed Quantum Dots for Single-Particle Tracking in Live Cells
title_short Compact and Blinking-Suppressed Quantum Dots for Single-Particle Tracking in Live Cells
title_sort compact and blinking-suppressed quantum dots for single-particle tracking in live cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4266335/
https://www.ncbi.nlm.nih.gov/pubmed/25157589
http://dx.doi.org/10.1021/jp5064325
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