Cargando…

Insulin renders diabetic rats resistant to acute ischemic stroke by arresting nitric oxide reaction with superoxide to form peroxynitrite

BACKGROUND: The functions of free radicals on the effects of insulin that result in protection against cerebral ischemic insult in diabetes remain undefined. This present study aims to explain the contradiction among nitric oxide (NO)/superoxide/peroxynitrite of insulin in amelioration of focal cere...

Descripción completa

Detalles Bibliográficos
Autores principales: Hung, Li-Man, Huang, Jiung-Pang, Liao, Jiuan-Miaw, Yang, Meng-Hsuan, Li, Dai-Er, Day, Yuan-Ji, Huang, Shiang-Suo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4266964/
https://www.ncbi.nlm.nih.gov/pubmed/25223305
http://dx.doi.org/10.1186/s12929-014-0092-0
_version_ 1782349086133846016
author Hung, Li-Man
Huang, Jiung-Pang
Liao, Jiuan-Miaw
Yang, Meng-Hsuan
Li, Dai-Er
Day, Yuan-Ji
Huang, Shiang-Suo
author_facet Hung, Li-Man
Huang, Jiung-Pang
Liao, Jiuan-Miaw
Yang, Meng-Hsuan
Li, Dai-Er
Day, Yuan-Ji
Huang, Shiang-Suo
author_sort Hung, Li-Man
collection PubMed
description BACKGROUND: The functions of free radicals on the effects of insulin that result in protection against cerebral ischemic insult in diabetes remain undefined. This present study aims to explain the contradiction among nitric oxide (NO)/superoxide/peroxynitrite of insulin in amelioration of focal cerebral ischemia–reperfusion (FC I/R) injury in streptozotocin (STZ)-diabetic rats and to delineate the underlying mechanisms. Long-Evans male rats were divided into three groups (age-matched controls, diabetic, and diabetic treated with insulin) with or without being subjected to FC I/R injury. RESULTS: Hyperglycemia exacerbated microvascular functions, increased cerebral NO production, and aggravated FC I/R-induced cerebral infarction and neurological deficits. Parallel with hypoglycemic effects, insulin improved microvascular functions and attenuated FC I/R injury in STZ-diabetic rats. Diabetes decreased the efficacy of NO and superoxide production, but NO and superoxide easily formed peroxynitrite in diabetic rats after FC I/R injury. Insulin treatment significantly rescued the phenomenon. CONCLUSIONS: These results suggest that insulin renders diabetic rats resistant to acute ischemic stroke by arresting NO reaction with superoxide to form peroxynitrite.
format Online
Article
Text
id pubmed-4266964
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-42669642014-12-16 Insulin renders diabetic rats resistant to acute ischemic stroke by arresting nitric oxide reaction with superoxide to form peroxynitrite Hung, Li-Man Huang, Jiung-Pang Liao, Jiuan-Miaw Yang, Meng-Hsuan Li, Dai-Er Day, Yuan-Ji Huang, Shiang-Suo J Biomed Sci Research BACKGROUND: The functions of free radicals on the effects of insulin that result in protection against cerebral ischemic insult in diabetes remain undefined. This present study aims to explain the contradiction among nitric oxide (NO)/superoxide/peroxynitrite of insulin in amelioration of focal cerebral ischemia–reperfusion (FC I/R) injury in streptozotocin (STZ)-diabetic rats and to delineate the underlying mechanisms. Long-Evans male rats were divided into three groups (age-matched controls, diabetic, and diabetic treated with insulin) with or without being subjected to FC I/R injury. RESULTS: Hyperglycemia exacerbated microvascular functions, increased cerebral NO production, and aggravated FC I/R-induced cerebral infarction and neurological deficits. Parallel with hypoglycemic effects, insulin improved microvascular functions and attenuated FC I/R injury in STZ-diabetic rats. Diabetes decreased the efficacy of NO and superoxide production, but NO and superoxide easily formed peroxynitrite in diabetic rats after FC I/R injury. Insulin treatment significantly rescued the phenomenon. CONCLUSIONS: These results suggest that insulin renders diabetic rats resistant to acute ischemic stroke by arresting NO reaction with superoxide to form peroxynitrite. BioMed Central 2014-09-16 /pmc/articles/PMC4266964/ /pubmed/25223305 http://dx.doi.org/10.1186/s12929-014-0092-0 Text en © Hung et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Hung, Li-Man
Huang, Jiung-Pang
Liao, Jiuan-Miaw
Yang, Meng-Hsuan
Li, Dai-Er
Day, Yuan-Ji
Huang, Shiang-Suo
Insulin renders diabetic rats resistant to acute ischemic stroke by arresting nitric oxide reaction with superoxide to form peroxynitrite
title Insulin renders diabetic rats resistant to acute ischemic stroke by arresting nitric oxide reaction with superoxide to form peroxynitrite
title_full Insulin renders diabetic rats resistant to acute ischemic stroke by arresting nitric oxide reaction with superoxide to form peroxynitrite
title_fullStr Insulin renders diabetic rats resistant to acute ischemic stroke by arresting nitric oxide reaction with superoxide to form peroxynitrite
title_full_unstemmed Insulin renders diabetic rats resistant to acute ischemic stroke by arresting nitric oxide reaction with superoxide to form peroxynitrite
title_short Insulin renders diabetic rats resistant to acute ischemic stroke by arresting nitric oxide reaction with superoxide to form peroxynitrite
title_sort insulin renders diabetic rats resistant to acute ischemic stroke by arresting nitric oxide reaction with superoxide to form peroxynitrite
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4266964/
https://www.ncbi.nlm.nih.gov/pubmed/25223305
http://dx.doi.org/10.1186/s12929-014-0092-0
work_keys_str_mv AT hungliman insulinrendersdiabeticratsresistanttoacuteischemicstrokebyarrestingnitricoxidereactionwithsuperoxidetoformperoxynitrite
AT huangjiungpang insulinrendersdiabeticratsresistanttoacuteischemicstrokebyarrestingnitricoxidereactionwithsuperoxidetoformperoxynitrite
AT liaojiuanmiaw insulinrendersdiabeticratsresistanttoacuteischemicstrokebyarrestingnitricoxidereactionwithsuperoxidetoformperoxynitrite
AT yangmenghsuan insulinrendersdiabeticratsresistanttoacuteischemicstrokebyarrestingnitricoxidereactionwithsuperoxidetoformperoxynitrite
AT lidaier insulinrendersdiabeticratsresistanttoacuteischemicstrokebyarrestingnitricoxidereactionwithsuperoxidetoformperoxynitrite
AT dayyuanji insulinrendersdiabeticratsresistanttoacuteischemicstrokebyarrestingnitricoxidereactionwithsuperoxidetoformperoxynitrite
AT huangshiangsuo insulinrendersdiabeticratsresistanttoacuteischemicstrokebyarrestingnitricoxidereactionwithsuperoxidetoformperoxynitrite