Massively parallel determination and modeling of endonuclease substrate specificity

We describe the identification and characterization of novel homing endonucleases using genome database mining to identify putative target sites, followed by high throughput activity screening in a bacterial selection system. We characterized the substrate specificity and kinetics of these endonucle...

Descripción completa

Detalles Bibliográficos
Autores principales: Thyme, Summer B., Song, Yifan, Brunette, T. J., Szeto, Mindy D., Kusak, Lara, Bradley, Philip, Baker, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4267613/
https://www.ncbi.nlm.nih.gov/pubmed/25389263
http://dx.doi.org/10.1093/nar/gku1096

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4267613/
https://www.ncbi.nlm.nih.gov/pubmed/25389263
http://dx.doi.org/10.1093/nar/gku1096

Ejemplares similares