Cargando…

Massively parallel determination and modeling of endonuclease substrate specificity

We describe the identification and characterization of novel homing endonucleases using genome database mining to identify putative target sites, followed by high throughput activity screening in a bacterial selection system. We characterized the substrate specificity and kinetics of these endonucle...

Descripción completa

Detalles Bibliográficos
Autores principales:	Thyme, Summer B., Song, Yifan, Brunette, T. J., Szeto, Mindy D., Kusak, Lara, Bradley, Philip, Baker, David
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2014
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4267613/ https://www.ncbi.nlm.nih.gov/pubmed/25389263 http://dx.doi.org/10.1093/nar/gku1096

Ejemplares similares

Reprogramming homing endonuclease specificity through computational design and directed evolution
por: Thyme, Summer B., et al.
Publicado: (2014)

Progressive engineering of a homing endonuclease genome editing reagent for the murine X-linked immunodeficiency locus
por: Wang, Yupeng, et al.
Publicado: (2014)

Catalytic domain of restriction endonuclease BmrI as a cleavage module for engineering endonucleases with novel substrate specificities
por: Chan, Siu-hong, et al.
Publicado: (2007)

Substrate specificity of the MUS81-EME2 structure selective endonuclease
por: Pepe, Alessandra, et al.
Publicado: (2014)

Structural insights into the substrate specificity of the endonuclease activity of the influenza virus cap-snatching mechanism
por: Kumar, Gyanendra, et al.
Publicado: (2021)

A novel three-unit tRNA splicing endonuclease found in ultrasmall Archaea possesses broad substrate specificity
por: Fujishima, Kosuke, et al.
Publicado: (2011)

Observation of unpaired substrate DNA in the flap endonuclease-1 active site
por: David Finger, L., et al.
Publicado: (2013)

The RNA-splicing endonuclease from the euryarchaeaon Methanopyrus kandleri is a heterotetramer with constrained substrate specificity
por: Kaneta, Ayano, et al.
Publicado: (2018)

Substrate specificity of human apurinic/apyrimidinic endonuclease APE1 in the nucleotide incision repair pathway
por: Kuznetsova, Alexandra A, et al.
Publicado: (2018)

Modifying a covarying protein–DNA interaction changes substrate preference of a site-specific endonuclease
por: Laforet, Marc, et al.
Publicado: (2019)

Biochemical analysis of the substrate specificity and sequence preference of endonuclease IV from bacteriophage T4, a dC-specific endonuclease implicated in restriction of dC-substituted T4 DNA synthesis
por: Hirano, Nobutaka, et al.
Publicado: (2006)

Creating highly specific nucleases by fusion of active restriction endonucleases and catalytically inactive homing endonucleases
por: Fonfara, Ines, et al.
Publicado: (2012)

Endonuclease G preferentially cleaves 5-hydroxymethylcytosine-modified DNA creating a substrate for recombination
por: Robertson, Adam B., et al.
Publicado: (2014)

Substrate preference of Gen endonucleases highlights the importance of branched structures as DNA damage repair intermediates
por: Bellendir, Stephanie P., et al.
Publicado: (2017)

Natural zinc ribbon HNH endonucleases and engineered zinc finger nicking endonuclease
por: Xu, Shuang-yong, et al.
Publicado: (2013)

Comprehensive computational design of mCreI homing endonuclease cleavage specificity for genome engineering
por: Ulge, Umut Y., et al.
Publicado: (2011)

Characterization of the 5-hydroxymethylcytosine-specific DNA restriction endonucleases
por: Borgaro, Janine G., et al.
Publicado: (2013)

In vivo cleavage specificity of Trypanosoma brucei editosome endonucleases
por: Carnes, Jason, et al.
Publicado: (2017)

Bacteriophage T4 endonuclease II, a promiscuous GIY-YIG nuclease, binds as a tetramer to two DNA substrates
por: Lagerbäck, Pernilla, et al.
Publicado: (2009)

Increasing cleavage specificity and activity of restriction endonuclease KpnI
por: Vasu, Kommireddy, et al.
Publicado: (2013)

Endonuclease domain of non-LTR retrotransposons: loss-of-function mutants and modeling of the R2Bm endonuclease
por: Govindaraju, Aruna, et al.
Publicado: (2016)

Pso2 (SNM1) is a DNA structure-specific endonuclease
por: Tiefenbach, Tracy, et al.
Publicado: (2012)

Rational engineering of sequence specificity in R.MwoI restriction endonuclease
por: Skowronek, Krzysztof, et al.
Publicado: (2012)

The Type II restriction endonuclease MvaI has dual specificity
por: Stier, Ildikó, et al.
Publicado: (2010)

Homing endonuclease I-CreI derivatives with novel DNA target specificities
por: Rosen, Laura E., et al.
Publicado: (2006)

Targeting individual subunits of the FokI restriction endonuclease to specific DNA strands
por: Sanders, Kelly L., et al.
Publicado: (2009)

Rational engineering of type II restriction endonuclease DNA binding and cleavage specificity
por: Morgan, Richard D., et al.
Publicado: (2009)

The Mus81-Mms4 structure-selective endonuclease requires nicked DNA junctions to undergo conformational changes and bend its DNA substrates for cleavage
por: Mukherjee, Sucheta, et al.
Publicado: (2014)

Thermodynamics of DNA target site recognition by homing endonucleases
por: Eastberg, Jennifer H., et al.
Publicado: (2007)

Type I restriction endonucleases are true catalytic enzymes
por: Bianco, Piero R., et al.
Publicado: (2009)

Restriction glycosylases: involvement of endonuclease activities in the restriction process
por: Zhang, Yingbiao, et al.
Publicado: (2017)

Structure-guided sequence specificity engineering of the modification-dependent restriction endonuclease LpnPI
por: Sasnauskas, Giedrius, et al.
Publicado: (2015)

Engineering domain fusion chimeras from I-OnuI family LAGLIDADG homing endonucleases
por: Baxter, Sarah, et al.
Publicado: (2012)

High-resolution profiling of homing endonuclease binding and catalytic specificity using yeast surface display
por: Jarjour, Jordan, et al.
Publicado: (2009)

EXOG, a novel paralog of Endonuclease G in higher eukaryotes
por: Cymerman, Iwona A., et al.
Publicado: (2008)

Dynamics and consequences of DNA looping by the FokI restriction endonuclease
por: Catto, Lucy E., et al.
Publicado: (2008)

Altered target site specificity variants of the I-PpoI His-Cys box homing endonuclease
por: Eklund, Jennifer L., et al.
Publicado: (2007)

Characterization of EndoTT, a novel single-stranded DNA-specific endonuclease from Thermoanaerobacter tengcongensis
por: Zhang, Shuli, et al.
Publicado: (2010)

Structure and specificity of the RNA-guided endonuclease Cas9 during DNA interrogation, target binding and cleavage
por: Josephs, Eric A., et al.
Publicado: (2015)

Structure-specific endonuclease activity of SNM1A enables processing of a DNA interstrand crosslink
por: Buzon, Beverlee, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_nddmok4jk0j8me873p2oslp9vc