Landscape of target:guide homology effects on Cas9-mediated cleavage

To study target sequence specificity, selectivity, and reaction kinetics of Streptococcus pyogenes Cas9 activity, we challenged libraries of random variant targets with purified Cas9::guide RNA complexes in vitro. Cleavage kinetics were nonlinear, with a burst of initial activity followed by slower...

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Detalles Bibliográficos
Autores principales: Hua Fu, Becky Xu, Hansen, Loren L., Artiles, Karen L., Nonet, Michael L., Fire, Andrew Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4267615/
https://www.ncbi.nlm.nih.gov/pubmed/25399416
http://dx.doi.org/10.1093/nar/gku1102

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4267615/
https://www.ncbi.nlm.nih.gov/pubmed/25399416
http://dx.doi.org/10.1093/nar/gku1102

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