Programmable RNA recognition and cleavage by CRISPR/Cas9

The CRISPR-associated protein Cas9 is an RNA-guided DNA endonuclease that uses RNA:DNA complementarity to identify target sites for sequence-specific doublestranded DNA (dsDNA) cleavage(1-5). In its native context, Cas9 acts on DNA substrates exclusively because both binding and catalysis require re...

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Detalles Bibliográficos
Autores principales: O’Connell, Mitchell R., Oakes, Benjamin L., Sternberg, Samuel H., East-Seletsky, Alexandra, Kaplan, Matias, Doudna, Jennifer A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268322/
https://www.ncbi.nlm.nih.gov/pubmed/25274302
http://dx.doi.org/10.1038/nature13769

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268322/
https://www.ncbi.nlm.nih.gov/pubmed/25274302
http://dx.doi.org/10.1038/nature13769

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