Cargando…

Programmable RNA recognition and cleavage by CRISPR/Cas9

The CRISPR-associated protein Cas9 is an RNA-guided DNA endonuclease that uses RNA:DNA complementarity to identify target sites for sequence-specific doublestranded DNA (dsDNA) cleavage(1-5). In its native context, Cas9 acts on DNA substrates exclusively because both binding and catalysis require re...

Descripción completa

Detalles Bibliográficos
Autores principales:	O’Connell, Mitchell R., Oakes, Benjamin L., Sternberg, Samuel H., East-Seletsky, Alexandra, Kaplan, Matias, Doudna, Jennifer A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2014
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268322/ https://www.ncbi.nlm.nih.gov/pubmed/25274302 http://dx.doi.org/10.1038/nature13769

Ejemplares similares

Conformational control of DNA target cleavage by CRISPR–Cas9
por: Sternberg, Samuel H., et al.
Publicado: (2015)

A conformational checkpoint between DNA binding and cleavage by CRISPR-Cas9
por: Dagdas, Yavuz S., et al.
Publicado: (2017)

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9
por: Sternberg, Samuel H., et al.
Publicado: (2014)

Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch
por: Oakes, Benjamin L, et al.
Publicado: (2016)

Enhanced proofreading governs CRISPR-Cas9 targeting accuracy
por: Chen, Janice S., et al.
Publicado: (2017)

Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9
por: Singh, Digvijay, et al.
Publicado: (2016)

Evolution of CRISPR RNA recognition and processing by Cas6 endonucleases
por: Niewoehner, Ole, et al.
Publicado: (2014)

Nucleosomes inhibit target cleavage by CRISPR-Cas9 in vivo
por: Yarrington, Robert M., et al.
Publicado: (2018)

A cleavage-based surrogate reporter for the evaluation of CRISPR–Cas9 cleavage efficiency
por: Jung, Soo Bin, et al.
Publicado: (2021)

PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage
por: Karvelis, Tautvydas, et al.
Publicado: (2020)

RNA-dependent RNA targeting by CRISPR-Cas9
por: Strutt, Steven C, et al.
Publicado: (2018)

Sequence features associated with the cleavage efficiency of CRISPR/Cas9 system
por: Liu, Xiaoxi, et al.
Publicado: (2016)

Structural insights into DNA cleavage activation of CRISPR-Cas9 system
por: Huai, Cong, et al.
Publicado: (2017)

Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs
por: Kundert, Kale, et al.
Publicado: (2019)

Disabling Cas9 by an anti-CRISPR DNA mimic
por: Shin, Jiyung, et al.
Publicado: (2017)

CRISPR/Cas9 editing of APP C-terminus attenuates β-cleavage and promotes α-cleavage
por: Sun, Jichao, et al.
Publicado: (2019)

Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules
por: Shams, Arik, et al.
Publicado: (2021)

Frequent Aneuploidy in Primary Human T Cells after CRISPR-Cas9 cleavage
por: Nahmad, A.D., et al.
Publicado: (2022)

PINCER: improved CRISPR/Cas9 screening by efficient cleavage at conserved residues
por: Veeneman, Brendan, et al.
Publicado: (2020)

Element coding based accurate evaluation of CRISPR/Cas9 initial cleavage
por: Hu, Jianyu, et al.
Publicado: (2021)

Machine learning in the estimation of CRISPR-Cas9 cleavage sites for plant system
por: Das, Jutan, et al.
Publicado: (2023)

Nucleosome breathing and remodeling constrain CRISPR-Cas9 function
por: Isaac, R Stefan, et al.
Publicado: (2016)

Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes
por: Watters, Kyle E., et al.
Publicado: (2020)

Editor's cut: DNA cleavage by CRISPR RNA-guided nucleases Cas9 and Cas12a
por: Swartjes, Thomas, et al.
Publicado: (2020)

CRISPR-Cas9 bends and twists DNA to read its sequence
por: Cofsky, Joshua C., et al.
Publicado: (2022)

Decorating chromatin for enhanced genome editing using CRISPR-Cas9
por: Chen, Evelyn, et al.
Publicado: (2022)

Structural basis for substrate recognition and cleavage by the dimerization-dependent CRISPR–Cas12f nuclease
por: Xiao, Renjian, et al.
Publicado: (2021)

Structural insights into target DNA recognition and cleavage by the CRISPR-Cas12c1 system
por: Zhang, Bo, et al.
Publicado: (2022)

CRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis B virus
por: Ramanan, Vyas, et al.
Publicado: (2015)

Biased and Unbiased Methods for the Detection of Off-Target Cleavage by CRISPR/Cas9: An Overview
por: Martin, Francisco, et al.
Publicado: (2016)

BoostMEC: predicting CRISPR-Cas9 cleavage efficiency through boosting models
por: Zarate, Oscar A., et al.
Publicado: (2022)

piCRISPR: Physically informed deep learning models for CRISPR/Cas9 off-target cleavage prediction
por: Störtz, Florian, et al.
Publicado: (2023)

Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery
por: Lin, Steven, et al.
Publicado: (2014)

Bipartite recognition of target RNAs activates DNA cleavage by the Type III-B CRISPR–Cas system
por: Elmore, Joshua R., et al.
Publicado: (2016)

DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle
por: Mikheikin, Andrey, et al.
Publicado: (2017)

Comparison of non-canonical PAMs for CRISPR/Cas9-mediated DNA cleavage in human cells
por: Zhang, Yilan, et al.
Publicado: (2014)

Permanent Inactivation of HBV Genomes by CRISPR/Cas9-Mediated Non-cleavage Base Editing
por: Yang, Yu-Chan, et al.
Publicado: (2020)

A CRISPR/Cas9 Cleavage System for Capturing Fungal Secondary Metabolite Gene Clusters
por: Xu, Xinran, et al.
Publicado: (2021)

Quantification of Cas9 binding and cleavage across diverse guide sequences maps landscapes of target engagement
por: Boyle, Evan A., et al.
Publicado: (2021)

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity
por: Pattanayak, Vikram, et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_ukg992k7plvi1fra2sk2k6rioq