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A fixed-point algorithm for estimating amplification efficiency from a polymerase chain reaction dilution series

BACKGROUND: The polymerase chain reaction amplifies and quantifies small amounts of DNA. It is a cyclic process, during each cycle of which each strand of template DNA is copied with probability approaching one: the amount of DNA approximately doubles and this amount can be estimated fluorimetricall...

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Detalles Bibliográficos
Autores principales: Jones, Michael E, Mayne, George C, Wang, Tingting, Watson, David I, Hussey, Damian J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268849/
https://www.ncbi.nlm.nih.gov/pubmed/25492416
http://dx.doi.org/10.1186/s12859-014-0372-4