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A fixed-point algorithm for estimating amplification efficiency from a polymerase chain reaction dilution series
BACKGROUND: The polymerase chain reaction amplifies and quantifies small amounts of DNA. It is a cyclic process, during each cycle of which each strand of template DNA is copied with probability approaching one: the amount of DNA approximately doubles and this amount can be estimated fluorimetricall...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268849/ https://www.ncbi.nlm.nih.gov/pubmed/25492416 http://dx.doi.org/10.1186/s12859-014-0372-4 |