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PiggyBac Mediated Multiplex Gene Transfer in Mouse Embryonic Stem Cell

PiggyBac system has been shown to have a high efficiency to mediate gene transfer. However, there are no reports on its efficiency to mediate multiplex transgenes in mouse embryonic stem cells. Here we first established an immortalized feeder cell line by introducing four antibiotic resistance genes...

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Detalles Bibliográficos
Autores principales: Lu, Xibin, Huang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4269400/
https://www.ncbi.nlm.nih.gov/pubmed/25517991
http://dx.doi.org/10.1371/journal.pone.0115072