Using a Non-Image-Based Medium-Throughput Assay for Screening Compounds Targeting N-myristoylation in Intracellular Leishmania Amastigotes

We have refined a medium-throughput assay to screen hit compounds for activity against N-myristoylation in intracellular amastigotes of Leishmania donovani. Using clinically-relevant stages of wild type parasites and an Alamar blue-based detection method, parasite survival following drug treatment o...

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Detalles Bibliográficos
Autores principales: Paape, Daniel, Bell, Andrew S., Heal, William P., Hutton, Jennie A., Leatherbarrow, Robin J., Tate, Edward W., Smith, Deborah F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4270692/
https://www.ncbi.nlm.nih.gov/pubmed/25522361
http://dx.doi.org/10.1371/journal.pntd.0003363

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4270692/
https://www.ncbi.nlm.nih.gov/pubmed/25522361
http://dx.doi.org/10.1371/journal.pntd.0003363

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