Preparation of Small RNAs Using Rolling Circle Transcription and Site-Specific RNA Disconnection

A facile and robust RNA preparation protocol was developed by combining rolling circle transcription (RCT) with RNA cleavage by RNase H. Circular DNA with a complementary sequence was used as the template for promoter-free transcription. With the aid of a 2′-O-methylated DNA, the RCT-generated tande...

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Detalles Bibliográficos
Autores principales: Wang, Xingyu, Li, Can, Gao, Xiaomeng, Wang, Jing, Liang, Xingguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4272408/
https://www.ncbi.nlm.nih.gov/pubmed/25584899
http://dx.doi.org/10.1038/mtna.2014.66

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4272408/
https://www.ncbi.nlm.nih.gov/pubmed/25584899
http://dx.doi.org/10.1038/mtna.2014.66

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