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Preparation of Small RNAs Using Rolling Circle Transcription and Site-Specific RNA Disconnection
A facile and robust RNA preparation protocol was developed by combining rolling circle transcription (RCT) with RNA cleavage by RNase H. Circular DNA with a complementary sequence was used as the template for promoter-free transcription. With the aid of a 2′-O-methylated DNA, the RCT-generated tande...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4272408/ https://www.ncbi.nlm.nih.gov/pubmed/25584899 http://dx.doi.org/10.1038/mtna.2014.66 |