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Performances of two different panfungal PCRs to detect mould DNA in formalin-fixed paraffin-embedded tissue: what are the limiting factors?

BACKGROUND: Detection of fungal DNA from formalin-fixed, paraffin-embedded (FFPE) tissue is challenging due to degradation of DNA and presence of PCR inhibitors in these samples. We analyzed FFPE samples of 26 patients by panfungal PCR and compared the results to the composite diagnosis according to...

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Detalles Bibliográficos
Autores principales: Babouee Flury, B, Weisser, M, Prince, S Savič, Bubendorf, L, Battegay, M, Frei, R, Goldenberger, D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4272807/
https://www.ncbi.nlm.nih.gov/pubmed/25518949
http://dx.doi.org/10.1186/s12879-014-0692-z