Dual sgRNA-directed gene knockout using CRISPR/Cas9 technology in Caenorhabditis elegans

The CRISPR RNA-guided Cas9 nuclease gene-targeting system has been successfully used for genome editing in a variety of organisms. Here, we report the use of dual sgRNA-guided Cas9 nuclease to generate knockout mutants of protein coding genes, noncoding genes, and repetitive sequences in C. elegans....

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Detalles Bibliográficos
Autores principales: Chen, Xiangyang, Xu, Fei, Zhu, Chengming, Ji, Jiaojiao, Zhou, Xufei, Feng, Xuezhu, Guang, Shouhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4273605/
https://www.ncbi.nlm.nih.gov/pubmed/25531445
http://dx.doi.org/10.1038/srep07581

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4273605/
https://www.ncbi.nlm.nih.gov/pubmed/25531445
http://dx.doi.org/10.1038/srep07581

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