Cargando…

Dual sgRNA-directed gene knockout using CRISPR/Cas9 technology in Caenorhabditis elegans

The CRISPR RNA-guided Cas9 nuclease gene-targeting system has been successfully used for genome editing in a variety of organisms. Here, we report the use of dual sgRNA-guided Cas9 nuclease to generate knockout mutants of protein coding genes, noncoding genes, and repetitive sequences in C. elegans....

Descripción completa

Detalles Bibliográficos
Autores principales:	Chen, Xiangyang, Xu, Fei, Zhu, Chengming, Ji, Jiaojiao, Zhou, Xufei, Feng, Xuezhu, Guang, Shouhong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2014
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4273605/ https://www.ncbi.nlm.nih.gov/pubmed/25531445 http://dx.doi.org/10.1038/srep07581

Ejemplares similares

Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA Ribonucleoproteins
por: Cho, Seung Woo, et al.
Publicado: (2013)

Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency
por: Dang, Ying, et al.
Publicado: (2015)

Targeted Chromosomal Rearrangements via Combinatorial Use of CRISPR/Cas9 and Cre/LoxP Technologies in Caenorhabditis elegans
por: Chen, Xiangyang, et al.
Publicado: (2018)

The efficient generation of knockout microglia cells using a dual-sgRNA strategy by CRISPR/Cas9
por: Zhang, Mengfei, et al.
Publicado: (2022)

Evaluation of CRISPR/Cas9 site-specific function and validation of sgRNA sequence by a Cas9/sgRNA-assisted reverse PCR technique
por: Zhang, Beibei, et al.
Publicado: (2021)

Different Effects of sgRNA Length on CRISPR-mediated Gene Knockout Efficiency
por: Zhang, Jian-Ping, et al.
Publicado: (2016)

Reliable CRISPR/Cas9 Genome Engineering in Caenorhabditis elegans Using a Single Efficient sgRNA and an Easily Recognizable Phenotype
por: El Mouridi, Sonia, et al.
Publicado: (2017)

Targeted genome engineering in Caenorhabditis elegans
por: Chen, Xiangyang, et al.
Publicado: (2016)

Targeting N-Terminal Huntingtin with a Dual-sgRNA Strategy by CRISPR/Cas9
por: Wu, Junjiao, et al.
Publicado: (2019)

Optimizing sgRNA to Improve CRISPR/Cas9 Knockout Efficiency: Special Focus on Human and Animal Cell
por: Shojaei Baghini, Sadegh, et al.
Publicado: (2021)

NGMA-2. Dual sgRNA-directed PD-L1 knockout in human glioblastoma cells using the CRISPR/Cas9 system
por: Fierro, Javier, et al.
Publicado: (2021)

De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens
por: He, Wei, et al.
Publicado: (2019)

Retraction: Optimizing sgRNA to improve CRISPR/Cas9 knockout efficiency: special focus on human and animal cell
Publicado: (2023)

Dual sgRNA‐directed gene deletion in basidiomycete Ganoderma lucidum using the CRISPR/Cas9 system
por: Liu, Ke, et al.
Publicado: (2020)

Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression
por: Long, Lu, et al.
Publicado: (2018)

Sequence determinants of improved CRISPR sgRNA design
por: Xu, Han, et al.
Publicado: (2015)

Author Correction: De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens
por: He, Wei, et al.
Publicado: (2020)

sgRNA Sequence Motifs Blocking Efficient CRISPR/Cas9-Mediated Gene Editing
por: Graf, Robin, et al.
Publicado: (2019)

A simple and rapid method for enzymatic synthesis of CRISPR-Cas9 sgRNA libraries
por: Yates, Joshua D, et al.
Publicado: (2021)

TransCRISPR–sgRNA design tool for CRISPR/Cas9 experiments targeting specific sequence motifs
por: Woźniak, Tomasz, et al.
Publicado: (2023)

A quick guide to CRISPR sgRNA design tools
por: Brazelton, Vincent A, et al.
Publicado: (2016)

Clinically Relevant Correction of Recessive Dystrophic Epidermolysis Bullosa by Dual sgRNA CRISPR/Cas9-Mediated Gene Editing
por: Bonafont, Jose, et al.
Publicado: (2019)

CRISPR/Cas9 Methodology for the Generation of Knockout Deletions in Caenorhabditis elegans
por: Au, Vinci, et al.
Publicado: (2018)

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
por: Doench, John G., et al.
Publicado: (2016)

Refined sgRNA efficacy prediction improves large- and small-scale CRISPR–Cas9 applications
por: Labuhn, Maurice, et al.
Publicado: (2018)

Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping
por: Gee, Peter, et al.
Publicado: (2020)

EpiCas-DL: Predicting sgRNA activity for CRISPR-mediated epigenome editing by deep learning
por: Yang, Qianqian, et al.
Publicado: (2022)

CRISPR/Cas9-mediated gene knockout is insensitive to target copy number but is dependent on guide RNA potency and Cas9/sgRNA threshold expression level
por: Yuen, Garmen, et al.
Publicado: (2017)

Dual-sgRNA CRISPR/Cas9 knockout of PD-L1 in human U87 glioblastoma tumor cells inhibits proliferation, invasion, and tumor-associated macrophage polarization
por: Fierro, Javier, et al.
Publicado: (2022)

A systematic evaluation of nucleotide properties for CRISPR sgRNA design
por: Kuan, Pei Fen, et al.
Publicado: (2017)

Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice
por: Jiang, Wenzhi, et al.
Publicado: (2013)

Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression
por: Radzisheuskaya, Aliaksandra, et al.
Publicado: (2016)

C-RNNCrispr: Prediction of CRISPR/Cas9 sgRNA activity using convolutional and recurrent neural networks
por: Zhang, Guishan, et al.
Publicado: (2020)

CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci
por: Fu, Yi, et al.
Publicado: (2016)

A Molecular Chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions
por: Cheng, Jijun, et al.
Publicado: (2016)

Evaluation of sgRNA Target Sites for CRISPR-Mediated Repression of TP53
por: Lawhorn, Ingrid E. B., et al.
Publicado: (2014)

Engineering Cellular Signal Sensors based on CRISPR-sgRNA Reconstruction Approaches
por: Zhan, Hengji, et al.
Publicado: (2020)

Establishment of a novel hepatic steatosis cell model by Cas9/sgRNA-mediated DGKθ gene knockout
por: Zhang, Jingjing, et al.
Publicado: (2018)

Optimized Cas9:sgRNA delivery efficiently generates biallelic MSTN knockout sheep without affecting meat quality
por: Zhou, Shiwei, et al.
Publicado: (2022)

Targeted Genome Editing of Sweet Orange Using Cas9/sgRNA
por: Jia, Hongge, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_066r552ji3ndl2ud51te0n2kb2