Antibody Stabilization of Peptide–MHC Multimers Reveals Functional T Cells Bearing Extremely Low-Affinity TCRs

Fluorochrome-conjugated peptide–MHC (pMHC) multimers are commonly used in combination with flow cytometry for direct ex vivo visualization and characterization of Ag-specific T cells, but these reagents can fail to stain cells when TCR affinity and/or TCR cell-surface density are low. pMHC multimer...

Descripción completa

Detalles Bibliográficos
Autores principales: Tungatt, Katie, Bianchi, Valentina, Crowther, Michael D., Powell, Wendy E., Schauenburg, Andrea J., Trimby, Andrew, Donia, Marco, Miles, John J., Holland, Christopher J., Cole, David K., Godkin, Andrew J., Peakman, Mark, Straten, Per Thor, Svane, Inge Marie, Sewell, Andrew K., Dolton, Garry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AAI 2015
Materias:
Novel Immunological Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4273996/
https://www.ncbi.nlm.nih.gov/pubmed/25452566
http://dx.doi.org/10.4049/jimmunol.1401785

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4273996/
https://www.ncbi.nlm.nih.gov/pubmed/25452566
http://dx.doi.org/10.4049/jimmunol.1401785

Ejemplares similares