Cargando…

Antibody Stabilization of Peptide–MHC Multimers Reveals Functional T Cells Bearing Extremely Low-Affinity TCRs

Fluorochrome-conjugated peptide–MHC (pMHC) multimers are commonly used in combination with flow cytometry for direct ex vivo visualization and characterization of Ag-specific T cells, but these reagents can fail to stain cells when TCR affinity and/or TCR cell-surface density are low. pMHC multimer...

Descripción completa

Detalles Bibliográficos
Autores principales:	Tungatt, Katie, Bianchi, Valentina, Crowther, Michael D., Powell, Wendy E., Schauenburg, Andrea J., Trimby, Andrew, Donia, Marco, Miles, John J., Holland, Christopher J., Cole, David K., Godkin, Andrew J., Peakman, Mark, Straten, Per Thor, Svane, Inge Marie, Sewell, Andrew K., Dolton, Garry
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	AAI 2015
Materias:	Novel Immunological Methods
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4273996/ https://www.ncbi.nlm.nih.gov/pubmed/25452566 http://dx.doi.org/10.4049/jimmunol.1401785

Ejemplares similares

Enhanced Detection of Antigen-Specific CD4(+) T Cells Using Altered Peptide Flanking Residue Peptide–MHC Class II Multimers
por: Holland, Christopher J., et al.
Publicado: (2015)

Peptide–MHC Class I Tetramers Can Fail To Detect Relevant Functional T Cell Clonotypes and Underestimate Antigen-Reactive T Cell Populations
por: Rius, Cristina, et al.
Publicado: (2018)

Optimized Peptide–MHC Multimer Protocols for Detection and Isolation of Autoimmune T-Cells
por: Dolton, Garry, et al.
Publicado: (2018)

Engineering of Isogenic Cells Deficient for MR1 with a CRISPR/Cas9 Lentiviral System: Tools To Study Microbial Antigen Processing and Presentation to Human MR1-Restricted T Cells
por: Laugel, Bruno, et al.
Publicado: (2016)

TCR‐induced alteration of primary MHC peptide anchor residue
por: Madura, Florian, et al.
Publicado: (2019)

Structural basis for ineffective T-cell responses to MHC anchor residue-improved “heteroclitic” peptides
por: Madura, Florian, et al.
Publicado: (2015)

T-cell libraries allow simple parallel generation of multiple peptide-specific human T-cell clones
por: Theaker, Sarah M., et al.
Publicado: (2016)

MHC class I multimers
por: Sun, Mei-Yi, et al.
Publicado: (2001)

Discovery of CD8 T cell epitopes in Merkel cell polyomavirus through combinatorial encoding with MHC multimers
por: Lyngaa, Rikke, et al.
Publicado: (2013)

TCR/pMHC Optimized Protein crystallization Screen
por: Bulek, Anna M., et al.
Publicado: (2012)

MHC Multimer: A Molecular Toolbox for Immunologists
por: Chang, Jun
Publicado: (2021)

Comparison of peptide–major histocompatibility complex tetramers and dextramers for the identification of antigen-specific T cells
por: Dolton, G, et al.
Publicado: (2014)

Genome-wide CRISPR-Cas9 Screening Reveals Ubiquitous T cell Cancer Targeting via the monomorphic MHC class I related protein MR1
por: Crowther, Michael D., et al.
Publicado: (2020)

Production and Thermal Exchange of Conditional Peptide‐MHC I Multimers
por: Luimstra, Jolien J., et al.
Publicado: (2019)

Hotspot autoimmune T cell receptor binding underlies pathogen and insulin peptide cross-reactivity
por: Cole, David K., et al.
Publicado: (2016)

Minimal conformational plasticity enables TCR cross-reactivity to different MHC class II heterodimers
por: Holland, Christopher J., et al.
Publicado: (2012)

Re-Directing CD4(+) T Cell Responses with the Flanking Residues of MHC Class II-Bound Peptides: The Core is Not Enough
por: Holland, Christopher J., et al.
Publicado: (2013)

Human leukocyte antigen (HLA) class II peptide flanking residues tune the immunogenicity of a human tumor-derived epitope
por: MacLachlan, Bruce J., et al.
Publicado: (2019)

Potent T cell agonism mediated by a very rapid TCR/pMHC interaction
por: Boulter, Jonathan M, et al.
Publicado: (2007)

Protein kinase inhibitors substantially improve the physical detection of T-cells with peptide-MHC tetramers
por: Lissina, Anna, et al.
Publicado: (2009)

Public T-Cell Receptors (TCRs) Revisited by Analysis of the Magnitude of Identical and Highly-Similar TCRs in Virus-Specific T-Cell Repertoires of Healthy Individuals
por: Huisman, Wesley, et al.
Publicado: (2022)

The TCR’s sensitivity to self-peptide–MHC dictates the ability of naïve CD8(+) T cells to respond to foreign antigens
por: Fulton, Ross B., et al.
Publicado: (2014)

Real time detection of peptide–MHC dissociation reveals that improvement of primary MHC-binding residues can have a minimal, or no, effect on stability
por: Miles, Kim M., et al.
Publicado: (2011)

TCRs alive on the periphery
por: Tuma, Rabiya S.
Publicado: (2005)

Analysis of Vδ1 T cells in clinical grade melanoma-infiltrating lymphocytes
por: Donia, Marco, et al.
Publicado: (2012)

A Versatile Simple Capture Assay for Assessing the Structural Integrity of MHC Multimer Reagents
por: Reed, Brendan K., et al.
Publicado: (2015)

Turn to TCRs when CARs fail
por: Stauss, Hans J.
Publicado: (2017)

Cryopreservation of MHC Multimers: Recommendations for Quality Assurance in Detection of Antigen Specific T Cells
por: Hadrup, Sine Reker, et al.
Publicado: (2015)

Optimization in Detection of Antigen‐Specific T Cells Through Differentially Labeled MHC Multimers
por: Pedersen, Natasja Wulff, et al.
Publicado: (2019)

Simultaneous Detection of Circulating Autoreactive CD8(+) T-Cells Specific for Different Islet Cell–Associated Epitopes Using Combinatorial MHC Multimers
por: Velthuis, Jurjen H., et al.
Publicado: (2010)

Dual Molecular Mechanisms Govern Escape at Immunodominant HLA A2-Restricted HIV Epitope
por: Cole, David K., et al.
Publicado: (2017)

Structural Basis for Self-Discrimination by Neoantigen-Specific TCRs
por: Finnigan, John P., et al.
Publicado: (2023)

A flexible MHC class I multimer loading system for large-scale detection of antigen-specific T cells
por: Luimstra, Jolien J., et al.
Publicado: (2018)

Metabolic Adaptation of Human CD4(+) and CD8(+) T-Cells to T-Cell Receptor-Mediated Stimulation
por: Jones, Nicholas, et al.
Publicado: (2017)

New Methods for Direct Delivery of Chemotherapy for Treating Brain Tumors
por: Sawyer, Andrew J., et al.
Publicado: (2007)

Structural Mechanism Underpinning Cross-reactivity of a CD8(+) T-cell Clone That Recognizes a Peptide Derived from Human Telomerase Reverse Transcriptase
por: Cole, David K., et al.
Publicado: (2017)

Molecular constraints on CDR3 for thymic selection of MHC-restricted TCRs from a random pre-selection repertoire
por: Lu, Jinghua, et al.
Publicado: (2019)

Genetic and Structural Basis for Selection of a Ubiquitous T Cell Receptor Deployed in Epstein-Barr Virus Infection
por: Miles, John J., et al.
Publicado: (2010)

Nonstimulatory peptide–MHC enhances human T-cell antigen-specific responses by amplifying proximal TCR signaling
por: Zhao, Xiang, et al.
Publicado: (2018)

A Single Autoimmune T Cell Receptor Recognizes More Than a Million Different Peptides
por: Wooldridge, Linda, et al.
Publicado: (2012)

Cannot write session to /tmp/vufind_sessions/sess_ucojllkkfrag418drsn6ijkqgq