Facile and high-efficient immobilization of histidine-tagged multimeric protein G on magnetic nanoparticles

This work reports the high-efficient and one-step immobilization of multimeric protein G on magnetic nanoparticles. The histidine-tagged (His-tag) recombinant multimeric protein G was overexpressed in Escherichia coli BL21 by the repeated linking of protein G monomers with a flexible linker. High-ef...

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Detalles Bibliográficos
Autores principales: Lee, Jiho, Chang, Jeong Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2014
Materias:
Nano Express
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4277612/
https://www.ncbi.nlm.nih.gov/pubmed/25593554
http://dx.doi.org/10.1186/1556-276X-9-664

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4277612/
https://www.ncbi.nlm.nih.gov/pubmed/25593554
http://dx.doi.org/10.1186/1556-276X-9-664

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