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Facile and high-efficient immobilization of histidine-tagged multimeric protein G on magnetic nanoparticles
This work reports the high-efficient and one-step immobilization of multimeric protein G on magnetic nanoparticles. The histidine-tagged (His-tag) recombinant multimeric protein G was overexpressed in Escherichia coli BL21 by the repeated linking of protein G monomers with a flexible linker. High-ef...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4277612/ https://www.ncbi.nlm.nih.gov/pubmed/25593554 http://dx.doi.org/10.1186/1556-276X-9-664 |