Hot Fusion: An Efficient Method to Clone Multiple DNA Fragments as Well as Inverted Repeats without Ligase

Molecular cloning is utilized in nearly every facet of biological and medical research. We have developed a method, termed Hot Fusion, to efficiently clone one or multiple DNA fragments into plasmid vectors without the use of ligase. The method is directional, produces seamless junctions and is not...

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Detalles Bibliográficos
Autores principales: Fu, Changlin, Donovan, William P., Shikapwashya-Hasser, Olga, Ye, Xudong, Cole, Robert H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4281135/
https://www.ncbi.nlm.nih.gov/pubmed/25551825
http://dx.doi.org/10.1371/journal.pone.0115318

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4281135/
https://www.ncbi.nlm.nih.gov/pubmed/25551825
http://dx.doi.org/10.1371/journal.pone.0115318

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