Construction of a recombinant lentivirus containing human microRNA-7-3 and its inhibitory effects on glioma proliferation☆

In the present study, we constructed a lentivirus, FIV-CMV-GFP-miR-7-3, containing the microRNA-7-3 gene and the green fluorescent protein gene, and used it to transfect human glioma U251 cells. Fluorescence microscopy showed that 80% of U251 cells expressed green fluorescence. Real-time reverse tra...

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Detalles Bibliográficos
Autores principales: Dong, Lun, Han, Chongxu, Zhang, Hengzhu, Gu, Xuewen, Li, Jian, Wu, Yongkang, Wang, Xiaodong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Techniques and Methods: Emerging Technology in Neural Regeneration
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4281417/
https://www.ncbi.nlm.nih.gov/pubmed/25558228
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.27.009

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4281417/
https://www.ncbi.nlm.nih.gov/pubmed/25558228
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.27.009

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