Integration of Host Strain Bioengineering and Bioprocess Development Using Ultra-Scale Down Studies to Select the Optimum Combination: An Antibody Fragment Primary Recovery Case Study

An ultra scale-down primary recovery sequence was established for a platform E. coli Fab production process. It was used to evaluate the process robustness of various bioengineered strains. Centrifugal discharge in the initial dewatering stage was determined to be the major cause of cell breakage. T...

Descripción completa

Detalles Bibliográficos
Autores principales: Aucamp, Jean P, Davies, Richard, Hallet, Damien, Weiss, Amanda, Titchener-Hooker, Nigel J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2014
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282095/
https://www.ncbi.nlm.nih.gov/pubmed/24838387
http://dx.doi.org/10.1002/bit.25259

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282095/
https://www.ncbi.nlm.nih.gov/pubmed/24838387
http://dx.doi.org/10.1002/bit.25259

Ejemplares similares