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Integration of Host Strain Bioengineering and Bioprocess Development Using Ultra-Scale Down Studies to Select the Optimum Combination: An Antibody Fragment Primary Recovery Case Study
An ultra scale-down primary recovery sequence was established for a platform E. coli Fab production process. It was used to evaluate the process robustness of various bioengineered strains. Centrifugal discharge in the initial dewatering stage was determined to be the major cause of cell breakage. T...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BlackWell Publishing Ltd
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282095/ https://www.ncbi.nlm.nih.gov/pubmed/24838387 http://dx.doi.org/10.1002/bit.25259 |