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EasyClone: method for iterative chromosomal integration of multiple genes in Saccharomyces cerevisiae
Development of strains for efficient production of chemicals and pharmaceuticals requires multiple rounds of genetic engineering. In this study, we describe construction and characterization of EasyClone vector set for baker's yeast Saccharomyces cerevisiae, which enables simultaneous expressio...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BlackWell Publishing Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282123/ https://www.ncbi.nlm.nih.gov/pubmed/24151867 http://dx.doi.org/10.1111/1567-1364.12118 |