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EasyClone: method for iterative chromosomal integration of multiple genes in Saccharomyces cerevisiae

Development of strains for efficient production of chemicals and pharmaceuticals requires multiple rounds of genetic engineering. In this study, we describe construction and characterization of EasyClone vector set for baker's yeast Saccharomyces cerevisiae, which enables simultaneous expressio...

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Detalles Bibliográficos
Autores principales:	Jensen, Niels B, Strucko, Tomas, Kildegaard, Kanchana R, David, Florian, Maury, Jérôme, Mortensen, Uffe H, Forster, Jochen, Nielsen, Jens, Borodina, Irina
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BlackWell Publishing Ltd 2014
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282123/ https://www.ncbi.nlm.nih.gov/pubmed/24151867 http://dx.doi.org/10.1111/1567-1364.12118

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