A protocol for the subcellular fractionation of Saccharomyces cerevisiae using nitrogen cavitation and density gradient centrifugation

Most protocols for yeast subcellular fractionation involve the use of mechanical shear forces to lyse the spheroplasts produced by the enzymatic digestion of the Saccharomyces cerevisiae cell wall. These mechanical homogenization procedures often involve the manual use of devices such as the Dounce...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Yuchong, Lilley, Kathryn S, Oliver, Stephen G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2014
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282465/
https://www.ncbi.nlm.nih.gov/pubmed/24510422
http://dx.doi.org/10.1002/yea.3002

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282465/
https://www.ncbi.nlm.nih.gov/pubmed/24510422
http://dx.doi.org/10.1002/yea.3002

Ejemplares similares