Site-directed mutagenesis in Arabidopsis thaliana using dividing tissue-targeted RGEN of the CRISPR/Cas system to generate heritable null alleles

MAIN CONCLUSION: Dividing tissue-targeted site-directed mutagenesis using RGEN of CRISPR/Cas system produces heritable mutations in Arabidopsis thaliana. ABSTRACT: Site-directed genome engineering in higher plants has great potential for basic research and molecular breeding. Here, we describe a met...

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Detalles Bibliográficos
Autores principales: Hyun, Youbong, Kim, Jungeun, Cho, Seung Woo, Choi, Yeonhee, Kim, Jin-Soo, Coupland, George
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2014
Materias:
Emerging Technologies
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282705/
https://www.ncbi.nlm.nih.gov/pubmed/25269397
http://dx.doi.org/10.1007/s00425-014-2180-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282705/
https://www.ncbi.nlm.nih.gov/pubmed/25269397
http://dx.doi.org/10.1007/s00425-014-2180-5

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