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Site-directed mutagenesis in Arabidopsis thaliana using dividing tissue-targeted RGEN of the CRISPR/Cas system to generate heritable null alleles
MAIN CONCLUSION: Dividing tissue-targeted site-directed mutagenesis using RGEN of CRISPR/Cas system produces heritable mutations in Arabidopsis thaliana. ABSTRACT: Site-directed genome engineering in higher plants has great potential for basic research and molecular breeding. Here, we describe a met...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer Berlin Heidelberg
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4282705/ https://www.ncbi.nlm.nih.gov/pubmed/25269397 http://dx.doi.org/10.1007/s00425-014-2180-5 |