A phytochemical comparison of saw palmetto products using gas chromatography and (1)H nuclear magnetic resonance spectroscopy metabolomic profiling
OBJECTIVES: Preparations containing saw palmetto berries are used in the treatment of benign prostatic hyperplasia (BPH). There are many products on the market, and relatively little is known about their chemical variability and specifically the composition and quality of different saw palmetto prod...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BlackWell Publishing Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284019/ https://www.ncbi.nlm.nih.gov/pubmed/24417505 http://dx.doi.org/10.1111/jphp.12198 |
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author | Booker, Anthony Suter, Andy Krnjic, Ana Strassel, Brigitte Zloh, Mire Said, Mazlina Heinrich, Michael |
author_facet | Booker, Anthony Suter, Andy Krnjic, Ana Strassel, Brigitte Zloh, Mire Said, Mazlina Heinrich, Michael |
author_sort | Booker, Anthony |
collection | PubMed |
description | OBJECTIVES: Preparations containing saw palmetto berries are used in the treatment of benign prostatic hyperplasia (BPH). There are many products on the market, and relatively little is known about their chemical variability and specifically the composition and quality of different saw palmetto products notwithstanding that in 2000, an international consultation paper from the major urological associations from the five continents on treatments for BPH demanded further research on this topic. Here, we compare two analytical approaches and characterise 57 different saw palmetto products. METHODS: An established method – gas chromatography – was used for the quantification of nine fatty acids, while a novel approach of metabolomic profiling using (1)H nuclear magnetic resonance (NMR) spectroscopy was used as a fingerprinting tool to assess the overall composition of the extracts. KEY FINDINGS: The phytochemical analysis determining the fatty acids showed a high level of heterogeneity of the different products in the total amount and of nine single fatty acids. A robust and reproducible (1)H NMR spectroscopy method was established, and the results showed that it was possible to statistically differentiate between saw palmetto products that had been extracted under different conditions but not between products that used a similar extraction method. Principal component analysis was able to determine those products that had significantly different metabolites. CONCLUSIONS: The metabolomic approach developed offers novel opportunities for quality control along the value chain of saw palmetto and needs to be followed further, as with this method, the complexity of a herbal extract can be better assessed than with the analysis of a single group of constituents. |
format | Online Article Text |
id | pubmed-4284019 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BlackWell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-42840192015-01-14 A phytochemical comparison of saw palmetto products using gas chromatography and (1)H nuclear magnetic resonance spectroscopy metabolomic profiling Booker, Anthony Suter, Andy Krnjic, Ana Strassel, Brigitte Zloh, Mire Said, Mazlina Heinrich, Michael J Pharm Pharmacol Research Papers OBJECTIVES: Preparations containing saw palmetto berries are used in the treatment of benign prostatic hyperplasia (BPH). There are many products on the market, and relatively little is known about their chemical variability and specifically the composition and quality of different saw palmetto products notwithstanding that in 2000, an international consultation paper from the major urological associations from the five continents on treatments for BPH demanded further research on this topic. Here, we compare two analytical approaches and characterise 57 different saw palmetto products. METHODS: An established method – gas chromatography – was used for the quantification of nine fatty acids, while a novel approach of metabolomic profiling using (1)H nuclear magnetic resonance (NMR) spectroscopy was used as a fingerprinting tool to assess the overall composition of the extracts. KEY FINDINGS: The phytochemical analysis determining the fatty acids showed a high level of heterogeneity of the different products in the total amount and of nine single fatty acids. A robust and reproducible (1)H NMR spectroscopy method was established, and the results showed that it was possible to statistically differentiate between saw palmetto products that had been extracted under different conditions but not between products that used a similar extraction method. Principal component analysis was able to determine those products that had significantly different metabolites. CONCLUSIONS: The metabolomic approach developed offers novel opportunities for quality control along the value chain of saw palmetto and needs to be followed further, as with this method, the complexity of a herbal extract can be better assessed than with the analysis of a single group of constituents. BlackWell Publishing Ltd 2014-06 2014-01-13 /pmc/articles/PMC4284019/ /pubmed/24417505 http://dx.doi.org/10.1111/jphp.12198 Text en © 2014 The Authors. Journal of Pharmacy and Pharmacology published by John Wiley & Sons Ltd on behalf of Royal Pharmaceutical Society. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Papers Booker, Anthony Suter, Andy Krnjic, Ana Strassel, Brigitte Zloh, Mire Said, Mazlina Heinrich, Michael A phytochemical comparison of saw palmetto products using gas chromatography and (1)H nuclear magnetic resonance spectroscopy metabolomic profiling |
title | A phytochemical comparison of saw palmetto products using gas chromatography and (1)H nuclear magnetic resonance spectroscopy metabolomic profiling |
title_full | A phytochemical comparison of saw palmetto products using gas chromatography and (1)H nuclear magnetic resonance spectroscopy metabolomic profiling |
title_fullStr | A phytochemical comparison of saw palmetto products using gas chromatography and (1)H nuclear magnetic resonance spectroscopy metabolomic profiling |
title_full_unstemmed | A phytochemical comparison of saw palmetto products using gas chromatography and (1)H nuclear magnetic resonance spectroscopy metabolomic profiling |
title_short | A phytochemical comparison of saw palmetto products using gas chromatography and (1)H nuclear magnetic resonance spectroscopy metabolomic profiling |
title_sort | phytochemical comparison of saw palmetto products using gas chromatography and (1)h nuclear magnetic resonance spectroscopy metabolomic profiling |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284019/ https://www.ncbi.nlm.nih.gov/pubmed/24417505 http://dx.doi.org/10.1111/jphp.12198 |
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