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Rise-Time of FRET-Acceptor Fluorescence Tracks Protein Folding

Uniform labeling of proteins with fluorescent donor and acceptor dyes with an equimolar ratio is paramount for accurate determination of Förster resonance energy transfer (FRET) efficiencies. In practice, however, the labeled protein population contains donor-labeled molecules that have no correspon...

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Detalles Bibliográficos
Autores principales: Lindhoud, Simon, Westphal, Adrie H., van Mierlo, Carlo P. M., Visser, Antonie J. W. G., Borst, Jan Willem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284793/
https://www.ncbi.nlm.nih.gov/pubmed/25535076
http://dx.doi.org/10.3390/ijms151223836