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Rise-Time of FRET-Acceptor Fluorescence Tracks Protein Folding
Uniform labeling of proteins with fluorescent donor and acceptor dyes with an equimolar ratio is paramount for accurate determination of Förster resonance energy transfer (FRET) efficiencies. In practice, however, the labeled protein population contains donor-labeled molecules that have no correspon...
Autores principales: | Lindhoud, Simon, Westphal, Adrie H., van Mierlo, Carlo P. M., Visser, Antonie J. W. G., Borst, Jan Willem |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284793/ https://www.ncbi.nlm.nih.gov/pubmed/25535076 http://dx.doi.org/10.3390/ijms151223836 |
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