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Parallel tagged amplicon sequencing of transcriptome-based genetic markers for Triturus newts with the Ion Torrent next-generation sequencing platform

Next-generation sequencing is a fast and cost-effective way to obtain sequence data for nonmodel organisms for many markers and for many individuals. We describe a protocol through which we obtain orthologous markers for the crested newts (Amphibia: Salamandridae: Triturus), suitable for analysis of...

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Detalles Bibliográficos
Autores principales: Wielstra, B, Duijm, E, Lagler, P, Lammers, Y, Meilink, W R M, Ziermann, J M, Arntzen, J W
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4285185/
https://www.ncbi.nlm.nih.gov/pubmed/24571307
http://dx.doi.org/10.1111/1755-0998.12242