Increased proteome coverage by combining PAGE and peptide isoelectric focusing: Comparative study of gel-based separation approaches

The in-depth analysis of complex proteome samples requires fractionation of the sample into subsamples prior to LC-MS/MS in shotgun proteomics experiments. We have established a 3D workflow for shotgun proteomics that relies on protein separation by 1D PAGE, gel fractionation, trypsin digestion, and...

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Detalles Bibliográficos
Autores principales: Atanassov, Ilian, Urlaub, Henning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2013
Materias:
Technology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4285200/
https://www.ncbi.nlm.nih.gov/pubmed/23943586
http://dx.doi.org/10.1002/pmic.201300035

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4285200/
https://www.ncbi.nlm.nih.gov/pubmed/23943586
http://dx.doi.org/10.1002/pmic.201300035

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