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Increased proteome coverage by combining PAGE and peptide isoelectric focusing: Comparative study of gel-based separation approaches
The in-depth analysis of complex proteome samples requires fractionation of the sample into subsamples prior to LC-MS/MS in shotgun proteomics experiments. We have established a 3D workflow for shotgun proteomics that relies on protein separation by 1D PAGE, gel fractionation, trypsin digestion, and...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BlackWell Publishing Ltd
2013
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4285200/ https://www.ncbi.nlm.nih.gov/pubmed/23943586 http://dx.doi.org/10.1002/pmic.201300035 |