Affinity maturation of Cry1Aa toxin to the Bombyx mori cadherin-like receptor by directed evolution based on phage display and biopanning selections of domain II loop 2 mutant toxins

Directed evolution of a Cry1Aa toxin using phage display and biopanning was performed to generate an increased binding affinity to the Bombyx mori cadherin-like receptor (BtR175). Three mutant toxins ((371)WGLA(374), (371)WPHH(374), (371)WRPQ(374)25) with 16-, 16-, and 50-fold higher binding affinit...

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Detalles Bibliográficos
Autores principales: Endo, Haruka, Kobayashi, Yuki, Hoshino, Yasushi, Tanaka, Shiho, Kikuta, Shingo, Tabunoki, Hiroko, Sato, Ryoichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2014
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287183/
https://www.ncbi.nlm.nih.gov/pubmed/25044375
http://dx.doi.org/10.1002/mbo3.188

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287183/
https://www.ncbi.nlm.nih.gov/pubmed/25044375
http://dx.doi.org/10.1002/mbo3.188

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