CTAB-mediated, single-step preparation of competent Escherichia coli, Bifidobacterium sp. and Kluyveromyces lactis cells

An efficient and reproducible method for transformation depends on the competency of the organism. We have developed a simple method for the preparation of competent Escherichia coli, Kluyveromyces lactis, and Bifidobacterium sp. by using a buffer containing cetyl trimethyl ammonium bromide (CTAB) a...

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Detalles Bibliográficos
Autores principales: Rajagopal, Kammara, Singh, Praveen Kumar, Kumar, Rajesh, Siddiqui, Kaneez Fatima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287956/
https://www.ncbi.nlm.nih.gov/pubmed/25606463
http://dx.doi.org/10.1016/j.mgene.2014.10.002

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287956/
https://www.ncbi.nlm.nih.gov/pubmed/25606463
http://dx.doi.org/10.1016/j.mgene.2014.10.002

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