Extendable blocking probe in reverse transcription for analysis of RNA variants with superior selectivity

Here we provide the first strategy to use a competitive Extendable Blocking Probe (ExBP) for allele-specific priming with superior selectivity at the stage of reverse transcription. In order to analyze highly similar RNA variants, a reverse-transcriptase primer whose sequence matches a specific vari...

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Detalles Bibliográficos
Autores principales: Ho, Tho H., Dang, Kien X., Lintula, Susanna, Hotakainen, Kristina, Feng, Lin, Olkkonen, Vesa M., Verschuren, Emmy W., Tenkanen, Tuomas, Haglund, Caj, Kolho, Kaija-Leena, Stenman, Ulf-Hakan, Stenman, Jakob
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4288146/
https://www.ncbi.nlm.nih.gov/pubmed/25378315
http://dx.doi.org/10.1093/nar/gku1048

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4288146/
https://www.ncbi.nlm.nih.gov/pubmed/25378315
http://dx.doi.org/10.1093/nar/gku1048

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