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An optimized kit-free method for making strand-specific deep sequencing libraries from RNA fragments

Deep sequencing of strand-specific cDNA libraries is now a ubiquitous tool for identifying and quantifying RNAs in diverse sample types. The accuracy of conclusions drawn from these analyses depends on precise and quantitative conversion of the RNA sample into a DNA library suitable for sequencing....

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Detalles Bibliográficos
Autores principales: Heyer, Erin E., Ozadam, Hakan, Ricci, Emiliano P., Cenik, Can, Moore, Melissa J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4288154/
https://www.ncbi.nlm.nih.gov/pubmed/25505164
http://dx.doi.org/10.1093/nar/gku1235