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An optimized kit-free method for making strand-specific deep sequencing libraries from RNA fragments
Deep sequencing of strand-specific cDNA libraries is now a ubiquitous tool for identifying and quantifying RNAs in diverse sample types. The accuracy of conclusions drawn from these analyses depends on precise and quantitative conversion of the RNA sample into a DNA library suitable for sequencing....
Autores principales: | Heyer, Erin E., Ozadam, Hakan, Ricci, Emiliano P., Cenik, Can, Moore, Melissa J. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4288154/ https://www.ncbi.nlm.nih.gov/pubmed/25505164 http://dx.doi.org/10.1093/nar/gku1235 |
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