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iPhos: a toolkit to streamline the alkaline phosphatase-assisted comprehensive LC-MS phosphoproteome investigation

BACKGROUND: Comprehensive characterization of the phosphoproteome in living cells is critical in signal transduction research. But the low abundance of phosphopeptides among the total proteome in cells remains an obstacle in mass spectrometry-based proteomic analysis. To provide a solution, an alter...

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Detalles Bibliográficos
Autores principales: Yang, Tzu-Hsien, Chang, Hong-Tsun, Hsiao, Eric SL, Sun, Juo-Ling, Wang, Chung-Ching, Wu, Hsin-Yi, Liao, Pao-Chi, Wu, Wei-Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4290636/
https://www.ncbi.nlm.nih.gov/pubmed/25521246
http://dx.doi.org/10.1186/1471-2105-15-S16-S10