Restriction Cascade Exponential Amplification (RCEA) assay with an attomolar detection limit: a novel, highly specific, isothermal alternative to qPCR

An alternative to qPCR was developed for nucleic acid assays, involving signal rather than target amplification. The new technology, Restriction Cascade Exponential Amplification (RCEA), relies on specific cleavage of probe-target hybrids by restriction endonucleases (REase). Two mutant REases for a...

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Detalles Bibliográficos
Autores principales: Ghindilis, Andrey L., Smith, Maria W., Simon, Holly M., Seoudi, Ihab A., Yazvenko, Nina S., Murray, Iain A., Fu, Xiaoqing, Smith, Kenneth, Jen-Jacobson, Linda, Xu, Shuang-yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4291554/
https://www.ncbi.nlm.nih.gov/pubmed/25583452
http://dx.doi.org/10.1038/srep07737

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4291554/
https://www.ncbi.nlm.nih.gov/pubmed/25583452
http://dx.doi.org/10.1038/srep07737

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