Transcription Activator-Like Effector Nuclease (TALEN)-Mediated CLYBL Targeting Enables Enhanced Transgene Expression and One-Step Generation of Dual Reporter Human Induced Pluripotent Stem Cell (iPSC) and Neural Stem Cell (NSC) Lines

Targeted genome engineering to robustly express transgenes is an essential methodology for stem cell-based research and therapy. Although designer nucleases have been used to drastically enhance gene editing efficiency, targeted addition and stable expression of transgenes to date is limited at sing...

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Detalles Bibliográficos
Autores principales: Cerbini, Trevor, Funahashi, Ray, Luo, Yongquan, Liu, Chengyu, Park, Kyeyoon, Rao, Mahendra, Malik, Nasir, Zou, Jizhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4294658/
https://www.ncbi.nlm.nih.gov/pubmed/25587899
http://dx.doi.org/10.1371/journal.pone.0116032

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